Actin polymerization in platelets from patients with WAS and XLT and from controls.
(A, B) Platelets isolated from whole blood and maintained at 37°C in autologous platelet-rich plasma were stimulated with either PGF2α, ADP, or buffer (▪)at the indicated concentrations.21 Data reflect the typical responses from each patient group and the control group. Error bars represent the SEM for duplicate simultaneous determinations for a single donor (control, WAS, or XLT). These graphs are representative of data collected from 2 patients with WAS (○), 2 patients with XLT (⧫), and 5 control donors (▴). (C-F) Representative scanning electron micrographs of platelets fixed before (C, E) and 180 seconds after stimulation (D, F) with 1 μmol/L PGF2α from a patient with classic WAS (E, F) and control (C, D). Images are 8-μm fields, and samples were prepared as previously described.28 Platelets isolated from patients with WAS were consistently smaller, as previously reported.2 Images are representative of data collected from 2 patients with WAS and 3 control donors.
