Kinetics of actin polymerization in WAS, XLT, and control lymphocytes.

(A, B) Actin polymerization in lymphocytes in response to stimulation with 100 nmol/L PMA, 100 nmol/L bryostatin (Bryo), and a buffer control (▪). Error bars represent SEM of duplicate simultaneous determinations for a single donor (control, ▴; WAS, ○; or XLT, ⧫). These graphs are representative of data collected from 2 patients with WAS, 5 patients with XLT, and 5 control donors. (C) Actin polymerization in lymphocytes in response to stimulation with OKT3 anti-CD3-receptor antibody and a buffer control. Error bars represent SEM of duplicate simultaneous determinations for a single donor (control, WAS, or XLT). These graphs are representative of data collected from 1 patient with WAS, 1 patient with XLT, and 2 healthy volunteers.