Viability, apoptosis, and necrosis in WAS, XLT, and control lymphocytes isolated 24 hours after venipuncture.

Percentages of viable, apoptotic, and necrotic lymphocytes isolated from 24-hour-old WAS, XLT, and control blood were determined by annexin-V binding/propidium iodide exclusion. Lymphocytes were purified 24 hours after venipuncture by gradient centrifugation of partially purified whole blood. WAS data represent the average of 5 separate determinations performed in 2 patients with WAS. Control data represent the average of 5 experiments in 4 volunteers run concurrently with experiments in the patients with WAS. XLT data represent the average of 3 experiments in 3 patients. Error bars represent SEM.