IgG antidonor antibody production in BALB/c mice with weekly transfusions of 10⁶ allogeneic platelet-pulsed BALB/c APC.

Each panel represents overlaid fluorescence histograms of C57BL/6 WBC incubated with sera (1:25 dilution) from a representative transfused mouse and labeled with FITC-goat antimouse IgG. The front histogram in each panel represents the prebleed serum, and each of the subsequent histograms are consecutive transfusions (weeks 1-6). A shift of the fluorescence to the right represents presence of antibody. (A) 10⁶ unpulsed BALB/c APC transfused into BALB/c recipients. (B) 10⁶ BALB/c platelet-pulsed APC transfused into BALB/c recipients. (C) 10⁶ C57BL/6 platelet-pulsed APC transfused into BALB/c recipients.