Analysis of IgG isotype patterns.

Recipient mice were transfused with donor platelet-pulsed APC that were additionally exposed to inhibitors during the pulsing step (chloroquine, Brefeldin A, and MG115). Sera sampled at the second, fourth, and sixth transfusions were analyzed for total IgG, IgG1, and IgG2a antibodies by flow cytometry. Each data point represents the percent change in antibody titer compared to APC not exposed to inhibitor, that is, the mean IgG1 or IgG2a titers obtained in the inhibitor groups divided by the mean titers in the noninhibitor group (as shown in Figure 2).