Analysis of serum IL-4.

Recipient mice were transfused with donor platelet-pulsed APC that were additionally exposed to inhibitors during the pulsing step (chloroquine, Brefeldin A, and MG115). Sera at the first, second, and sixth transfusions were analyzed for IL-4 and IL-12 cytokines by ELISA. Data are expressed as the fold change in IL-4 and were calculated by dividing the mean pg/mL values obtained at each transfusion week with the mean pg/mL values in the prebleed sera. There were no significant changes in IL-12 levels.