Overexpression of GLO1 in apoptosis-resistant UK711 cells.

(A) Wild-type U937 (W) and the mutant UK711 (M) cells were incubated with 5 μmol/L of VP-16 for 6 hours or 0.6 μmol/L of ADM for 12 hours. After the harvest of the cells, nuclear DNA fragmentation was analyzed by 2% agarose gel electrophoresis. (B) Northern blot analysis was carried out with mRNAs prepared from U937 and UK711 cells. The RNAs were transferred to nylon membranes and probed with the GLO1gene fragment (upper panels). Blots were also rehybridized with a GAPDH probe as a control (lower panels). Expression of the mRNA was quantified by densitometric analysis.