American Society of Hematology

Fig. 1.

$Fig. 1. MEFV expression in bone marrow leukocytes and precursor cells. / (A). Ethidium bromide (EtBr)–stained polyacrylamide gel electrophoresis (PAGE) of reverse transcriptase–polymerase chain reaction (RT-PCR) products of MEFV and β-actin messenger RNAs (mRNAs) from unfractionated bone marrow cells (lane 1), bone marrow leukocytes (lane 2), bone marrow granulocytic cells (lane 3), and an enriched population of CD34+ peripheral blood hematopoietic precursors (PBHP; lane 4). (B) Wright-Giemsa–stained cytospin preparations of the cells used for these analyses. Original magnification ×400. (C) Photomicrographs of Wright- Giemsa–stained in situ hybridizations of bone marrow leukocytes. Results with use of a gene-specific MEFV antisense riboprobe (right panel) and a nonspecific MEFV sense strand control riboprobe (left panel) are shown. Original magnification ×400.$

MEFV expression in bone marrow leukocytes and precursor cells.

(A). Ethidium bromide (EtBr)–stained polyacrylamide gel electrophoresis (PAGE) of reverse transcriptase–polymerase chain reaction (RT-PCR) products of MEFV and β-actin messenger RNAs (mRNAs) from unfractionated bone marrow cells (lane 1), bone marrow leukocytes (lane 2), bone marrow granulocytic cells (lane 3), and an enriched population of CD34⁺ peripheral blood hematopoietic precursors (PBHP; lane 4). (B) Wright-Giemsa–stained cytospin preparations of the cells used for these analyses. Original magnification ×400. (C) Photomicrographs of Wright- Giemsa–stained in situ hybridizations of bone marrow leukocytes. Results with use of a gene-specific MEFV antisense riboprobe (right panel) and a nonspecific MEFV sense strand control riboprobe (left panel) are shown. Original magnification ×400.

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