MEFV expression in unstimulated and in vitro differentiated myelomonocytic cell lines.

(A) EtBr-stained PAGE of RT-PCR products of the MEFV, LF, MPO, and β-actin RNAs from resting untreated HL60 cells (lane 1) and cultured HL60 cells induced to differentiate toward the neutrophilic lineage with 1 or 10 μmol/L retinoic acid (lanes 2 and 3), toward the eosinophilic lineage with alkaline pH (pH 7.8; lane 4), and toward the monocytic lineage with phorbol ester. Positive and negative control PCR reactions using the MEFV-containing plasmid pV75-1 and water, respectively, are shown. (B) EtBr-stained PAGE of RT-PCR products of the MEFV and β-actin mRNAs from the monocytic cell lines U937 and THP-1.