Kinetics of engraftment of transduced CD34⁺cord blood cells in NOD/SCID mice.

NOD/SCID mice were transplanted with unsorted transduced cells, as described in Materials and methods. At different times after transplantation, BM samples from NOD/SCID recipients were obtained and the rate of engraftment and proportion of EGFP fluorescent cells determined by FACS analysis. Panels A and B show, respectively, data deduced from human CD45⁺ and CD34⁺ analysis (Table 2 presents BM codes). White bars represent the kinetics of engraftment of human cells, either expressing (•) or not expressing (○) the EGFP transgene. Dark bars represent the proportion of human cells expressing EGFP. The phenotypic characteristics of the different grafts are shown in Table 1. Data corresponding to last analyses are shown in Table 2.