Fig. 2.
DCs triggered through CD40 maintain an activated phenotype on a subsequent DEX exposure.
Immature DCs were activated with the CD8-CD40L fusion protein. DEX (10−6 mol/L) or medium control was added 48 hours later, and cells were analyzed after 2 additional days of culture. The comparison with immature DCs maintained in medium alone is shown. Empty histograms show the background staining with isotype controls monoclonal antibody, and solid histograms represent specific staining of the indicated cell-surface markers. Specific mean fluorescence intensities are indicated. Mean fluorescence intensities of isotype controls were between 3 and 5. Data are representative of 2 independent experiments.
