Fig. 1.
Effect of thrombin exposure time on B16F10 growth after 72 hours of culture.
One × 104 B16F10 cells were starved of FCS for 24 hours and then incubated in RPMI + 1% FCS with 1 U/mL thrombin. One aliquot (bar 2) was washed in RPMI + 1% FCS after 3 hours and then incubated for 72 hours in the wash media. Another aliquot (bar 3) was incubated for 72 hours with 1 U/mL thrombin. Control cells (bar 1) were incubated for 72 hours without thrombin. Optical density refers to color development with the MTT assay at 490 nm after incubation for 24 hours at 37°C in 5% CO2. SEM is given for the mean of quadruplicate measurements of 3 different cell clones.
