Thrombin induces cell cycle arrest at G₂M.

B16F10 (S14) and DU145 cells were incubated with various concentrations of thrombin for 36 hours, washed in PBS, and then assayed for propidium iodide binding via flow cytometry. The heavy line refers to the raw data. The shaded areas refer to the computer simulated peaks of the cell cycle from left to right at pre-G_o, G_o-G₁, and G₂M, respectively. The hatched areas refer to the S phase. The fine line refers to the sum of the raw data and the computer simulated peaks. The number on the far left refers to the percentage total pre-G_o cells measured. The 2 numbers on the right refer to the percentage distribution of cells in cycle for G_o-G₁ and G₂M, respectively. The G_o-G₁, G₂M, and S cells equal 100%.