Virus DNA in PBMCs after autologous transfusion.

Semiquantitative DNA-PCR was performed from fresh PBMCs. DNA from cell mixtures was used as control. For this purpose, herpesvirus saimiri–transformed human T cells were mixed with PHA/IL-2–stimulated human T cells at ratios of 0/10⁶, 1/10⁶, 10/10⁶, 10²/10⁶, and 10³/10⁶. The time points of the PBMC preparations after the first autologous transfusion are marked for day 2 (d2), day 4 (d4), and week 1 (w1) to week 16 (w16). PCR for 2 different virus genes, stpC and vIL-17, is shown for monkey Mm7067.