Detection of clonal TCR-γ rearrangement and expression of T-cell marker in the HRS cells of patient 1.

(A) Analysis of the amplificates obtained after single copy TCR-γ PCR in single HRS cells (lane 1-4), reactive T-cells (lane 5-7), buffer control (lane 8), and negative control (lane 9) (B-cell line Raji) (polyacrylamide gel stained with ethidium bromide). (B) and (C) Immunohistologic staining for perforin (B) and CD8 (C) (APAAP staining). (D) CD30 staining of a frozen section from which the single HRS cells or their nuclei were isolated. In the insert, a Hodgkin cell is shown following isolation of its nucleus by micromanipulation.