Detection of clonal TCR-γ rearrangement and expression of perforin in the Hodgkin and Reed-Sternberg cells of patient 2.

(A) Analysis of the amplificates obtained after single copy TCR-γ PCR in single HRS cells (lane 1-4), reactive T-cells (lane 5-7), buffer control (lane 8), and negative control (lane 9) (B-cell line Raji) (polyacrylamide gel stained with ethidium bromide). (B) and (C) Immunohistologic staining for perforin (B) and CD30 (C) (APAAP staining). Note the perifollicular and noncohesive growth pattern of the CD30⁺ tumor cells.