Expression of IL-12Rβ1 and IL-12Rβ2 on cytokine-primed NK cells.

Purified NK cells were incubated for 3 days in the absence or presence of IL-2. Expression of IL-12 receptor chains was then determined by flow cytometry. Representative histograms demonstrate the expression of IL-12Rβ1 and IL-12Rβ2 on NK cells cultured without IL-2 (A) or with IL-2 (B). In (C), NK cells cultured with IL-2 were incubated with IL-12 before antibody labeling. The shaded area represents the isotype negative control.