Direct PCR bisulphite sequencing profiles.

Electrophoretograms of the CpG region of the E-cad gene from CpG dinucleotides 11-17 show the T peaks (dotted line) and C peaks (solid line) only. Note the sequence given within each panel is that of the bisulphite-converted DNA, except for the CpG dinucleotides that are shown as CG. The estimated amounts of methylcytosine, calculated by measuring the peak height of the cytosine over the peak heights of the cytosine plus thymine, are shown under each dinucleotide, where − indicates 0% methylation, + 1% to 25% methylation, ++ 26% to 50% methylation, +++ 51% to 75% methylation, and ++++ 76% to 100% methylation. (A) 50% methylated control PCR reaction, as described in Warnecke et al.23 (B) AML patient R99, with methylation throughout the region. (C) Normal bone marrow control N43, with no methylation evident throughout the region.