Mobilization of CD34⁺lineage⁻ and Sca⁺ lineage⁻cells in mixed chimeras.

(A) Representative histogram of spleen cells isolated from CY-treated mice that were stained with antibodies against a cocktail of lineage markers and CD34. The percentage of CD34⁺lineage⁻ cells is shown. (B) Ly 5.1expression on CD34⁺ lineage⁻ cells that were identified in the previous histogram. The percentage of CD34⁺ lineage⁻ cells that expressLy5.1 is shown. (C, D) The absolute number of wild type (Ly5.1⁺) and G-CSFR–deficient (Ly5.1⁻) CD34⁺lineage⁻ or Sca⁺ lineage⁻cells present in the spleen of saline- or CY-treated mice is shown. Data represent the mean plus or minus SD.