: Representative microsatellite marker studies in recipient dogs at selected times.

(A) Dog E390 received 2-Gy total body irradiation (TBI) and dog leukocyte antigen (DLA)–identical marrow from a litter mate and was treated after grafting with mycophenolate mofetil and cyclosporine for 4 and 5 weeks, respectively. The first unmodified DLI (8.0 × 10⁷ CD3⁺ cells/kg of body weight) was given 14 weeks after transplantation and the second (10.8 × 10⁷ CD3⁺ cells/kg) was given 30 weeks after transplantation, without producing a significant change in mixed-chimerism status. In contrast, mHA-sensitized DLI (7.1 × 10⁷ CD3⁺ cells/kg) given 59 weeks after grafting resulted in rapid conversion to complete donor chimerism, which was sustained beyond 87 weeks after transplantation. (B) Dog E433 received the same transplant regimen, including DLA-identical litter-mate marrow, as dog E390 (above). Unmodified DLI was given twice without producing a change in mixed-chimerism status (data not shown here). Sixty weeks after allografting, mHA-sensitized DLI (8.5 × 10⁷ CD3⁺/kg) was given, resulting in a rapid increase in donor chimerism to 98%. Host chimerism persisted, and by 105 weeks after allografting, had increased to 15% in both the PBMC and granulocyte fractions.