Intracellular TGF-β1 in CLRPP.

(A) Flow cytometric detection of autocrine TGF-β1 in isolated CLRPP. Dotted lines represent the background fluorescence of isotypical controls; black histograms are the specific fluorescence generated by the presence of anti-TGF-β1 mAb (clone 9016.2). The numbers indicate the percentage of positive cells and in parentheses the mean fluorescence channel is indicated. Data are representative of 6 different experiments. (B) Western blot analysis of TGF-β1 protein in isolated CLRPP, showing the specific band of 14 kd.