Levels of bcl-2, p53, c-myc, and p21.

RT-PCR analysis of bcl-2, p21, c-myc, and p53 was performed on 72-hour cultured CLRPP in the presence (anti–TGF-beta1) or the absence (Ctrl CLRPP) of anti-TGF-β1 mAb (10 μg/mL). Panels are photographs of ethidium bromide–stained gels. (PHA-stim.LymP indicates PHA-stimulated lymphocytes used as positive control; Neg.ctrl, no-sample negative control). The lower band in all panels is aldolase-A, which was coamplified with the other specific products and used as an internal control. Data are representative of 4 experiments. Parallel cultures in the presence of irrelevant isotype-matched mouse immunoglobulins showed comparable results to those observed in control CLRPP cultures. The bar graph shows the mean values ± SD expressed as the adsorbance ratios between the specific RNA and aldolase-A RNA level observed from 4 different experiments. P > .05 for p21, c-myc, and p53 RNA levels; P < .0001 for bcl-2 RNA levels.