Fig. 10.
Fig. 10. Expression levels of GATA-1 and PU.1 transcription factors in CLRPP. / GATA-1 and PU.1 RNA levels were determined by RT-PCR of isolated, 72-hour cultured control (Ctrl CLRPP) and anti-TGF-β1 treated (10 μg/mL; anti-TGF-beta1) CLRPP. The RT-PCR products were separated on agarose gel, transferred to filter, and hybridized with the specific probes for GATA-1, PU.1, and β-actin. β-Actin was coamplified with the genes of interest as an internal control. Bar graph shows the RNA levels expressed as the mean ± SD adsorbance ratios between the RNA of interest and that of β-actin observed in 4 different experiments. Parallel cultures in the presence of irrelevant isotype-matched mouse immunoglobulins showed comparable results to those observed in control CLRPP cultures.P < .0001 for GATA-1 and PU.1 RNA levels of 72-hour cultured control and anti-TGF-β1–treated CLRPP.

Expression levels of GATA-1 and PU.1 transcription factors in CLRPP.

GATA-1 and PU.1 RNA levels were determined by RT-PCR of isolated, 72-hour cultured control (Ctrl CLRPP) and anti-TGF-β1 treated (10 μg/mL; anti-TGF-beta1) CLRPP. The RT-PCR products were separated on agarose gel, transferred to filter, and hybridized with the specific probes for GATA-1, PU.1, and β-actin. β-Actin was coamplified with the genes of interest as an internal control. Bar graph shows the RNA levels expressed as the mean ± SD adsorbance ratios between the RNA of interest and that of β-actin observed in 4 different experiments. Parallel cultures in the presence of irrelevant isotype-matched mouse immunoglobulins showed comparable results to those observed in control CLRPP cultures.P < .0001 for GATA-1 and PU.1 RNA levels of 72-hour cultured control and anti-TGF-β1–treated CLRPP.

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