Restored hyporesponsive T cells remain anergic.
(A) Primed control or hyporesponsive T cells (2 × 104), induced by different mAb combinations, were restimulated with the original stimulator PBMCs (1 × 105) in the presence of 12.5 U/mL exogenous IL-2. The proliferative response was examined on day 3. (B) Control and hyporesponsive T cells derived from a primary MLC were recovered with antigen in the presence or absence of exogenously added IL-2 and subsequently restimulated for the second time in a tertiary MLC. The3H incorporation of this first restimulation is shown in Table 1 (SE < 10%). Next, 2 × 104 recovered cells were restimulated for a second time with 1 × 105 stimulator PBMCs in the absence of IL-2. The proliferative response was examined on day 3. Results are expressed as mean and SE of quadruplicate measurements. Representative experiments are shown.
