Fig. 1.
Schematic of the flow apparatus used for exposing ECs to RBC perfusion.
The endothelial monolayers were mounted in the parallel plate flow chamber by applying vacuum to hold the glass slide, gasket, and polycarbonate base together. RBC suspensions were driven by a reciprocating dual syringe pump, which determined the flow rate. The glass reservoir was connected to a humidified mixture of 95% air and 5% CO2 to maintain the pH levels of the media and also to keep the media oxygenated.
