RT-PCR analysis of CLTCL-ALK and the sequence of the cloned CLTCL-ALK cDNA fusion junction.

(A) All samples show the expected 270 bp CLTCL-ALK RT-PCR product (M, size markers).Total RNA prepared from the 3 biopsy specimens (lane 1 and 2: axillary lymph nodes; lane 3: skin tumor) of case 1. RNA obtained from the NPM-ALK-positive SU-DHL-1 cell line was used as negative control (T−). RT-PCR was performed using 2 rounds of PCR. (B) Nucleotide and deduced amino acid sequences ofCLTCL-ALK cDNA. The arrow shows the translocation breakpoint. Nucleotides are numbered from the first nucleotide of the CLTCL transcript.