IL-4 and IL-13 induce P-selectin mRNA expression in HUVEC.

Data were obtained using a semiquantitative RT-PCR method as described in “Materials and methods” to determine mRNA expression for soluble and membrane (surface)-bound P-selectin mRNA after 48-hour culture in medium, IL-4, and IL-13. The figure represents a digitally scanned image of the agarose gel. P-selectin mRNA expression was referenced to the housekeeping gene GAPDH. The cytokines induced an approximate doubling in both membrane and soluble P-selectin mRNA expression with no change in GAPDH expression. Data shown are from 2 independent experiments.