Fig. 5.
Immunocytochemical analysis of AC133-derived adherent cells using the nickel-glucose peroxidase anti-peroxidase technique.
(A) Negative control (AC133-derived adherent cells cultured for 14 days in the presence of stem cell growth factor [SCGF] and vascular endothelial growth factor [VEGF]). (B-G) AC133-derived adherent cells after 14 days of culture with SCGF and VEGF and labeled with anti-CD34 monoclonal antibody (MoAb), anti-VE-Cadherin MoAb, anti-KDR MoAb, anti-Tie-2 polyclonal antibody, anti-Ulex europaeusagglutinin-1 MoAb, and anti-von Willebrand factor MoAb, respectively. Original magnification ×100.