Immunocytochemical analysis of colonies grown from day 8 cells in semisolid media.

Day 8 cells were cultured for 14 days in methycellulose supplemented with stem cell growth factor and vascular endothelial growth factor. Resulting colonies were then dissolved by fixation, transferred to glass slides, and stained with anti-von Willebrand factor monoclonal antibody. Original magnification ×100.