Southern blot analysis of a murine P1 clone containing several Ly-6 family members.

Lanes 1 and 2 are empty P1 vector DNA digested with BamHI orEcoRI. Lanes 3-10 are P1 clone 13561 DNA digested withBamHI, EcoRI, BamHI, and EcoRI,BglII, PstI, PstI, and EcoRI,HindIII, or XbaI. Fragments were separated on 1% agarose and transferred to nitrocellulose. The blot was hybridized with a probe for (A) mSca-1, then was stripped and rehybridized with a probe for (B) mSca-2. The mSca-1 probe detects 2 homologous DNA sequences, whereas the mSca-2 probe detects a single DNA sequence.