Fas-induced T-cell death in ALD patients and 15 normal donors.

Left panel shows T cells cultured for 6 or 21 days with IL-2 and treated with anti-Fas mAb; relative cell survival was assessed after 18 hours. Right panel shows day-21 T-cells that were treated with anti-Fas mAb. Cell apoptosis was assessed after 6 hours by annexin V staining. Results are expressed as percentage of annexin V⁺ cells in the Fas-treated well corrected for the percentage of annexin V⁺ cells in the Fas-untreated well. The percentage of annexin V⁺ cells in the Fas-untreated well was always less than 10%. Results from normal donors (controls) are shown as mean ± SD.