Fig. 2.
Amount of rvWF secreted by transiently transfected COS-7 cells.
The concentration of WT and mutant rvWF in conditioned media was determined by ELISA. Absorbance at 492 nm was converted to units of vWF:Ag using a standard curve generated from dilutions of a calibrated pool of normal plasma. The results represent the mean ± SD of 5 independent transfections.