Fig. 4.
Fig. 4. Multimeric analysis of rvWF. / Conditioned media samples containing WT or mutant rvWF were electrophoresed in SDS-1.5% agarose gels, and multimers were visualized with alkaline-phosphatase–conjugated anti-vWF polyclonal antibodies. (A) Lane 1, WT; lane 2, Tyr25; lane 3, Gln91; lane 4, Tyr25/Gln91; lane 5, Tyr25/WT. (B) lane 1, WT; lane 2, Phe95; lane 3, Phe95/Gln91; lane 4, Phe95/WT.

Multimeric analysis of rvWF.

Conditioned media samples containing WT or mutant rvWF were electrophoresed in SDS-1.5% agarose gels, and multimers were visualized with alkaline-phosphatase–conjugated anti-vWF polyclonal antibodies. (A) Lane 1, WT; lane 2, Tyr25; lane 3, Gln91; lane 4, Tyr25/Gln91; lane 5, Tyr25/WT. (B) lane 1, WT; lane 2, Phe95; lane 3, Phe95/Gln91; lane 4, Phe95/WT.

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