Inhibition of Rho proteins or p38 MAPK activity blocks LPS-dependent activation of an NF-κB–dependent reporter gene.

HUVECs transiently transfected with an NF-κB–luciferase construct were pretreated with 10 ng/mL TcdB-10463 for 1 hour or 100μM SB 202190 for 30 minutes as indicated. Expression of reporter gene was measured by chemiluminescence as described in “Materials and methods.” Data presented are mean ± SEM of 3 separate experiments.