Fig. 6.
BG and BCNU mediates strong selection pressure.
Fold enrichment for ΔMGMT+ CFU was calculated by dividing the total estimated number of ΔMGMT+ CFU at sacrifice by the number of ΔMGMT+ CFU infused. Within 1 × 105 bone marrow cells, we obtained approximately 200 CFU, which increased to 800 after 5-FU treatment. Because the CFU transduction efficiency was 75%, the total number of infused ΔMGMT+ CFU was 6000, 1500, and 300 in the 100, 25, and 5 × 104 cell populations, respectively. At sacrifice, we estimated 6 × 105 CFU present in 300 × 106 total mouse bone marrow cells. ▪, 5 × 104 cohort; ▴, 25 × 104cohort; •, 100 × 104 cohort.
