Flow cytometric analyses of AC133⁺CD34⁺ fetal liver, cord blood, and adult bone marrow cells with CD164 Mabs.

Dot plots showing MACS-selected CD34⁺ cells isolated from fetal liver, cord blood, or bone marrow triple-labeled with either CD34-PerCP, AC133-PE, and isotype negative-control Mabs, mIgG3, mIgM, or mIgG2a, plus FITC-conjugated anti-isotype secondary antibodies and the CD164 Mabs 103B2/9E10, 105A5, and N6B6 followed by FITC-conjugated anti-isotype secondary antibodies. Dot plots show displays of CD34-PerCP/PE, AC133-PE, or mIgG1-PE versus the CD164 epitopes, 103B2/9E10, 105A5, and N6B6, or their isotype-matched negative controls on CD34⁺ cells before or after gating on the CD34⁺ subsets. Cells were analyzed on a FACSCalibur using Cellquest software.