American Society of Hematology

Fig. 3.

$Fig. 3. CD164 epitopes are more highly expressed on the AC133hiCD34hiCD38lo/− fetal liver cell subset. / Dot plots show MACS-selected CD34+ cells isolated from fetal liver labeled with CD34-PerCP, CD38-APC, AC133-PE, the CD164 Mabs 103B2/9E10 (B), 105A5 (C), and N6B6 (D) or isotype negative-control Mabs mIgG3 (A), mIgM, or mIgG2a, plus FITC-conjugated anti-isotype secondary antibodies. Isotype matched negative-control mIgG1-PE, mIgG1-APC, and mIgG1-PerCP were used in place of AC133-PE, CD38-APC, and CD34-PerCP (not shown). CD34-PerCP–positive cells with intermediate to high forward scatter and low side angle scatter characteristics (fraction R1) were analyzed on a FACSCalibur and gated on the basis of the relative expression of CD34 and CD38 (fractions R2, R3, R4, R5). These fractions were then analyzed for the expression of AC133 and the CD164 epitopes (Table 1).$

CD164 epitopes are more highly expressed on the AC133^hiCD34^hiCD38^lo/− fetal liver cell subset.

Dot plots show MACS-selected CD34⁺cells isolated from fetal liver labeled with CD34-PerCP, CD38-APC, AC133-PE, the CD164 Mabs 103B2/9E10 (B), 105A5 (C), and N6B6 (D) or isotype negative-control Mabs mIgG3 (A), mIgM, or mIgG2a, plus FITC-conjugated anti-isotype secondary antibodies. Isotype matched negative-control mIgG1-PE, mIgG1-APC, and mIgG1-PerCP were used in place of AC133-PE, CD38-APC, and CD34-PerCP (not shown). CD34-PerCP–positive cells with intermediate to high forward scatter and low side angle scatter characteristics (fraction R1) were analyzed on a FACSCalibur and gated on the basis of the relative expression of CD34 and CD38 (fractions R2, R3, R4, R5). These fractions were then analyzed for the expression of AC133 and the CD164 epitopes (Table 1).

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