American Society of Hematology

Figure 2

$Figure 2.   Inhibitory effect of the patient's IgG on TPO-induced human megakaryocyte colony formation. / The megakaryocyte progenitor assay was performed in a soft agar medium containing recombinant human TPO or recombinant human IL-3 as described previously.8 Glycosylated recombinant human TPO or recombinant human interleukin-3 was preincubated with the patient's IgG fractions before therapy (January 10, 1996) or during therapy(October 14, 1997), and added to a soft agar culture containing nonadherent human bone marrow mononuclear cells. After 14 days of culture, megakaryocyte colonies were immunohistochemically stained and counted. IgG fraction (96/1/10) is indicated by □; IgG fraction (97/10/14) is indicated by ▧; and 0.1% bovine serum albumin is indicated by ▪.$

  Inhibitory effect of the patient's IgG on TPO-induced human megakaryocyte colony formation.

The megakaryocyte progenitor assay was performed in a soft agar medium containing recombinant human TPO or recombinant human IL-3 as described previously.8 Glycosylated recombinant human TPO or recombinant human interleukin-3 was preincubated with the patient's IgG fractions before therapy (January 10, 1996) or during therapy(October 14, 1997), and added to a soft agar culture containing nonadherent human bone marrow mononuclear cells. After 14 days of culture, megakaryocyte colonies were immunohistochemically stained and counted. IgG fraction (96/1/10) is indicated by □; IgG fraction (97/10/14) is indicated by ▧; and 0.1% bovine serum albumin is indicated by ▪.

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