Quantitative RNA analysis of VEGF mutant ES cell cultures.

PhosphorImager data from the gels in Figure 3 were manipulated to subtract background and to normalize to the β-actin protected signal in the same lane. Relative band densities for each probe in het (+/−) and null (−/−) lanes were then compared to (+/+) wild-type levels for that probe. Each result, except for the VEGF analysis, is a compilation of at least 3 experiments on at least 2 (and sometimes 3) different sets of RNA prepared on different days and at different passage numbers. In all cases, statistical analyses compared VEGF het and null RNA levels to the VEGF wild-type RNA levels for that probe. *P < .05; **P < .000 0001.