PECAM/SSEA-1 double-label immunofluorescent antibody staining of VEGF mutant ES cultures.

Day 8 cultures were fixed and stained with mouse antimouse SSEA-1 (red) and rabbit polyclonal antimouse PECAM (green) antibodies, along with the appropriate secondary antibodies. Images were photographed with the appropriate FITC (B, E, H) or rhodamine (C, F, I) filters or with a double exposure using both filters sequentially (A, D, G). (A-C) Wild-type (+/+) VEGF ES cultures. (D-F) Hemizygous (+/−) VEGF mutant ES cultures. (G-I) Null (−/−) VEGF mutant ES cultures. Magnification, 50×.