Aberrant p15 methylation in adult and childhood acute leukemias.

(A) p15 methylation patterns in acute biphenotypic leukemia (ABL; lanes 1 and 2) and acute myeloid leukemia (AML; subtypes M1, M2, M3, M4, M5, M6, and M7 in lanes 3-24). DNA was amplified by using p15MF and p15MR primers. HS-Sultan DNA (HS) served as a methylated control. The 1-kilobase (kb) ladder is the molecular-weight standard. (B, C) Aberrantly methylated p15 sequences in blood plasma from patients with acute leukemia on ethidium bromide-stained agarose gels and Southern blots. Plasma DNA samples from patients withoutp15 methylation in blood cells or bone marrow are shown in lanes 1 to 4; samples from patients with p15 methylation in blood cells or bone marrow are shown in lanes 5 to 10. (D) p16methylation status in patients with AML (lanes 1-5) and acute lymphoblastic leukemia (ALL; lanes 6-10). DNA was amplified by using p16MF and p16MR primers.