FACS analysis of FcR expression in transfected Ba/F3 cells.

In (A), expression of FcαR and a FcαR mutant, FcαR_R209D, was analyzed by flow cytometry. In (B), the effect of co-expression of Ras, phosphatidylinositol 3-kinase, PTEN, and gag constructs on FcαR_wt expression was analyzed. Polyclonal stable cell lines were tested for expression of FcαR with PE-labeled A59, a monoclonal antibody against FcαR (CD89). Levels of FcαR expression are indicated as relative fluorescence, and nontransfected Ba/F3 cells were used as a control in each panel (gray line).