Inhibition of protein kinase C (PKC) results in abolished immunoglobulin A (IgA) binding on interleukin 3 (IL-3) stimulation.

IL-3-starved Ba/F3_FcαR (A) or Ba/F3_FcαR (p110K227E) cells (B) were pretreated for 10 minutes with buffer (white), p70S6K inhibitor rapamycin (20 ng/mL; gray), or PKC inhibitors GF109203X (1μmol/L; black) or Ro-31-8220 (1μmol/L; arched) and subsequently incubated with or without IL-3 for 15 minutes. Binding of IgA beads to these cells was measured, and results are expressed as rosette index (number of beads/100 cells) and as means ± SE (n = 3).