Fig. 9.
Binding of Ang-1 isoforms to Tie-2 by ligand–receptor precipitation.
(A) In vitro translated radioactive proteins of isoform 1.5 kb (lane 1), 1.3 kb (lane 2), and 0.9 kb (lane 3) are shown. (B) In vitro translated proteins were incubated with Tie-2-Fc, 1 μg/mL, and precipitated with protein G beads. After washing and elution, samples were discriminated by SDS-PAGE plus autoradiography. Lane 1, 2, and 3 (1.5-, 1.3-, and 0.9-kb isoforms, respectively, with protein G beads alone). Lanes 4, 5, and 6 (1.5-, 1.3-, and 0.9-kb isoforms, respectively, with Tie-2-Fc plus protein G beads. No immunoprecipitation was noted with the 1.3-kb isoform (lane 5) or with control 35S-luciferase (Tie-2-Fc plus protein G beads; data not shown).
