Induction of autophosphorylation of Tie-2 receptor by Ang-1 isoforms.

HUVE cells were stimulated with conditioned media collected from Ang-1 isoforms (Ang-1.5 kb, lane 1; 1.3 kb, lane 2; or 0.9 kb, lane 3) in transfected COS cells. Cell lysates were immunoprecipitated with anti-Tie-2 antibody. Immunoprecipitates were analyzed by Western blot with antiphosphotyrosine antibody.