Fig. 3.
Fig. 3. Constitutive activation of Stat factors in TEL-JAK-transformed Ba/F3 cells. / Analyses of Stat DNA-binding activities in IL-3-independent Ba/F3 cells expressing the indicated constructs. As a control for Stat1, Stat3, and Stat5 DNA-binding activities, nontransfected Ba/F3 cells were starved for 5 hours, then stimulated with 10 ng/mL of mIL-3 for 10 minutes. Nuclear extracts were submitted to EMSA using the bovine β-casein (A) and m67SIE (B) probes. The specificity of the shifted complexes was assessed by the addition of a 100-fold molar excess of cold competitors. Complexes supershifted by specific anti-Stat factors antibodies are indicated by open arrows. Note that constitutive Stat1 and Stat3 activations detected in TEL-JAK3-expressing Ba/F3 cells were observed after prolonged exposure.

Constitutive activation of Stat factors in TEL-JAK-transformed Ba/F3 cells.

Analyses of Stat DNA-binding activities in IL-3-independent Ba/F3 cells expressing the indicated constructs. As a control for Stat1, Stat3, and Stat5 DNA-binding activities, nontransfected Ba/F3 cells were starved for 5 hours, then stimulated with 10 ng/mL of mIL-3 for 10 minutes. Nuclear extracts were submitted to EMSA using the bovine β-casein (A) and m67SIE (B) probes. The specificity of the shifted complexes was assessed by the addition of a 100-fold molar excess of cold competitors. Complexes supershifted by specific anti-Stat factors antibodies are indicated by open arrows. Note that constitutive Stat1 and Stat3 activations detected in TEL-JAK3-expressing Ba/F3 cells were observed after prolonged exposure.

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