Degradation of ¹²⁵I-TFPI^C127 by HepG2 cells.

HepG2 cells growing in petri dishes were incubated with 0.6 nmol of¹²⁵I-TFPI^C127 at 37°C for the indicated times. At each time point, the overlying medium was removed and proteins were precipitated by trichloroacetic acid (TCA). The TCA-soluble radioactivity (ie, degradation products) was determined. Degradation of bacterial recombinant ¹²⁵I-TFPI by HepG2 cells was performed in the identical manner and served as a control. Numbers represent the means of duplicate determinations. Total degraded¹²⁵I-TFPI at 4 hours was less than 10% input.