Purification and binding specificities of bscCD19 × CD3.

(A) Western blot analysis of the purified bscCD19 × CD3 construct detected by anti-HisTag antibodies. The molecular mass (kd) is indicated on the left; the μg protein applied on the top. (B) FACS analysis with the bscCD19 × CD3 on different CD19-positive B-cell lines (Daudi, Blin-1, BJAB, Raji, and SKW6.4), on CD3-positive Jurkat cells and primary human PBLs, and on the CD3- and CD19-negative plasmacytoma cell line L363. Broken lines are negative controls with the secondary antibody anti–HisTag-FITC alone.