Fig. 2.
T-cell proliferation and cytotoxic activity of bscCD19 × CD3.
(A) Standard 3H-thymidine proliferation assay with unstimulated primary human PBMCs before and after depletion of B cells by immunomagnetic beads. (B) Cytotoxicity of bscCD19 × CD3 in a51Cr-release assay with primary human PBLs and different B-cell lines (BJAB, Raji, Blin-1, SKW6.4, and Daudi) in the presence of 60 units per mL IL-2; the E-T ratio was 10:1 and the incubation time was 4 hours. The SD in all triplicates was below 5%.
