Fig. 5.
Effect of pertussis toxin (Ptx) on MAP kinase activation by eotaxin.
Eosinophils were preincubated with buffer or Ptx (100 ng/mL) for 1 hour and stimulated with eotaxin (10−8 mol/L) for 1 minute. Cytosolic extracts were used for Western blotting with antibodies against dual threonine-tyrosine–phosphorylated ERK2 (α-PT/PY-ERK1/2) (A) or dual threonine-tyrosine–phosphorylated p38 (α-PT/PY-p38) (B). Ptx inhibited the phosphorylation of both ERK2 and p38.
